Developing Herbicide Resistant Transgenic Maize by Biolistic method

Transformation technology is a research or breeding tool to improve maize is routinely used in most industrial and some specialized public laboratories. However, transformation of many inbred lines remains a challenging task, especially when using gene gun as the delivery method. Efficient methods for in vitro propagation, regeneration, and transformation of plants are of pivotal importance to both basic and applied research. While being the world's major food crops, cereals are among the most difficult to-handle plants in tissue culture which severely limits genetic engineering approaches. Here we report success in generating transgenic plants from two maize inbred lines using an particle bombardment mediated standard vector system to target maize immature embryo calli.