Isolation and Identification of Lactobacilli from Raw Cow Milk

Lactobacilli play a major role in fermented dairy products and also contribute to the therapeutic aspects of human health. Raw milk sample was used in this study to isolate and identify the Lactobacilli and to find out the incidence of Lactobacilli Biochemical i.e. IMViC tests, Sugar fermentation test were used for identification of isolates of Lactobacilli from raw cow milk. Lactobacilli were used to evaluate their antimicrobial effect against common pathogens, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli by using the disc diffusion method. The study was successful in isolation and identification from raw cow milk in local region of Agra. The result of the present study indicates that, raw cow milk are potential source of probiotic Lactobacilli.