ELISA (Enzyme-Linked Immunsorbent Assay) Practical Report

Essay aus dem Jahr 2015 im Fachbereich Biologie - Humanbiologie, , Sprache: Deutsch, Abstract: The Enzyme-Linked Immunosorbent assay (ELISA) is a test that is conducted to identify the substance under study with the help of antibodies and changing color elements. It is the "wet-lab" oriented analytic biochemistry assay that consume enzyme immunoassay to detect the existence of a substance (antigen) in a liquid sample. It is of great help in medicine and plant pathology as a diagnostic tool and quality control check. ELISA test involves one antibody according to the need of the test, where the antigen is immobilized on the solid plate. The method is done specifically and nonspecifically. The report involves the description and construction of two different ELISAs.